Explain the functions of enzymes in DNA replication.

Enzyme / Protein	Specific Function
DNA pol I	Removes RNA primer and replaces it with newly synthesized DNA.
DNA pol III	The main enzyme adds nucleotides in the 5′-3′ direction.
Helicase	It opens the DNA helix by breaking hydrogen bonds between the nitrogenous bases.
Ligase	Seals the gaps between the Okazaki fragments to create one continuous DNA strand
Primase	Synthesizes RNA primers needed to start replication
Sliding Clamp	Helps to hold the DNA polymerase in place when nucleotides are being added
Topoisomerase	Helps relieve the strain on DNA when unwinding by causing breaks, and then resealing the DNA